HPTLC Method in Determination of Guggulosterone Z from Leaf Extract of Tribulus terrestris Linn

Abstract

High Performance Thin Layer Chromatography (HPTLC) method is one of the best methods used in the analysis of herbal drugs. This technique is used to determine the quantity of bioactive compounds present in the extract. They have been utilized to analyze various types of natural products like alkaloids, flavonoids, steroids, terpenes, saponins, tannins etc. In this study, we have used HPTLC technique to estimate Guggulsterone Z from leaf extract of Tribulus terrestris Linn. In order to prepare the extracts, dry powder of Tribulus terrestris Linn leaves were extracted with solvent and these extracts were subjected to HPTLC analysis using mobile phase. The chromatographic plates silica gel 60F254 aluminium sheets of 20 cm x 10cm dimension were used for developing the samples. The plate was air-dried after completion of the run and observed under UV lamp. In the present research work, the results obtained determined the presence of Guggulsterone Z in Tribulus terrestris Linn by HPTLC method.