Future Journal of Pharmaceuticals and Health Sciences

Development and validation of an optimized RP-HPLC method for the concurrent analysis of decitabine and cedazuridine

2025-04-05T15:04:17+0530

Decitabine and cedazuridine were analysed simultaneously using a reverse-phase RP-HPLC technique that was refined and verified. A Kromosil C18 column (250 mm × 4.6 mm, 5 µm) was used to produce chromatographic separation under isocratic elution conditions. Phosphate buffer (pH 4.5) and methanol (20:80 v/v) made up the mobile phase. The buffer was made from potassium dihydrogen orthophosphate, and the pH was modified with orthophosphoric acid. A photodiode array (PDA) detector set at 254 nm was used for detection, and the flow rate was set at 1.0 mL/min. With a 20 µL injection volume and a 10-minute run period, the column was kept at room temperature.It was discovered that the linearity of cedazuridine and decitabine ranged from 1 to 5 ?g/mL and 100 to 500 ?g/mL, respectively.The coefficient of linear regression did not exceed 0.999.Decitabine as well as cedazuridine had respective precisions of 0.2% and 0.6%.Decitabine %RSD 0.2 and cedazuridine %RSD 0.1 have intermediate precision. In order to determine the % recovery for Decitabine (99.84%) and Cedazuridine (100.51%), the accuracy was assessed.LOD and LOQ were determined to be within the range.The findings on the validation parameters satisfied USP and ICH standards.It concluded that the procedure was straightforward, linear, accurate, and precise. It was discovered that the technique may be applied with a high degree of accuracy and precision in ordinary laboratory analysis.

S adenosyl L methionine's antioxidant and anti-inflammatory characteristics have a cardioprotective effect on Metoprolol-induced chronic heart failure in Wistar rats

2025-04-05T14:27:12+0530

Oxidative stress and myocardial inflammation are closely linked to the onset and progression of chronic heart failure (CHF), hypertrophy, and remodeling. S-adenosylmethionine (SAMe), a dietary supplement with anti-inflammatory and antioxidant properties, has been shown to enhance ventricular remodeling by regulating angiogenesis and fibrosis. This study aimed to evaluate the cardioprotective effects of SAMe in Metoprolol (MPL)-induced CHF and its anti-inflammatory and antioxidant properties.Following animal ethics approval, CHF was induced in 24 Wistar rats by administering MPL (10 mg/kg) for 14 consecutive days. The rats were divided into four groups: Sham Control, Disease Control (DC), MPL + SAMe 100 mg, and MPL + SAMe 200 mg. Evaluations included histology, PET imaging, 18F-FDG biodistribution in heart tissue, glutathione (GSH) and tumor necrosis factor ? (TNF-?) levels, and heart-to-body weight ratio (HW/BW mg/g).SAMe significantly reduced HW/BW compared to DC (P < 0.001) and decreased 18F-FDG uptake in CHF-induced rats (P < 0.001). TNF-? and GSH levels were markedly elevated in both SAMe-treated groups (P < 0.001). Histology showed reduced inflammation and necrosis. SAMe demonstrated cardioprotective effects in MPL-induced CHF due to its anti-inflammatory and antioxidant properties.

Development and validation of a reverse-phase high-performance liquid chromatography method for indacaterol and budesonide analysis

2025-04-05T14:51:48+0530

An efficient, precise, and accurate technique for the concurrent estimation of compounds Indacaterol and Budesonide in pharmaceutical preparations were developed. The analysis utilized an Xterra C18 (4.6 × 150 mm, 5.0 µm) chromatographic column used in combination with a mobile phase ratio of buffer (0.1% orthophosphoric acid): acetonitrile (40:60% v/v) and a flow rate is 1 mL/min. The detection was performed at a wavelength of 220 nm. The recorded retention times for Indacaterol and Budesonide were 3.124 minutes and 4.270 minutes, respectively. Respectively. The percentage recovery was 100.01% for Indacaterol and 100.34% for Budesonide. The method demonstrated limits of detection (LOD) of 0.3 µg/mL and 0.6 µg/mL and limits of quantification (LOQ) of 1.0 µg/mL and 1.9 µg/mL for Indacaterol and Budesonide. Regression equations confirmed linearity within the established range. Stress degradation studies (acid, base, peroxide, thermal, and photostability conditions) indicated that the percentage degradation of Indacaterol and Budesonide remained within acceptable limits, ensuring method robustness.