HPTLC Method in Determination of Lupeol from Bark Extract of Crataeva religiosa Hook and Frost

Abstract

HPTLC (high performance thin-layer chromatographic) is a new method which has been developed for the determination of lupeol in petroleum ether extract from bark of Crateava religiosa. This method is simple, precise, rapid and selective. It can be applied to determine lupeol in petroleum ether extract from bark of Crateava religiosa. This method is suitable for routine analysis and can be used by anyone with minimal training. As per International Conference on Harmonization (ICH) guidelines we have applied different concentrations of lupeol as standard on HPTLC plates for the quantification of lupeol from the petroleum ether extract in bark of Crateava religiosa. The retention factor of lupeol was found to be 0.43f. The developed and validated HPTLC method was employed for lupeol in petroleum ether extract from bark of Crateava religiosa for standardization of the content of the marker. The results obtained in the validation assays are promising, with an average error of less than 0.5%. The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of lupeol in petroleum ether extract from bark of Crateava religiosa.