Synthesis and Characterization of Siver Nano-Particles Prepared from Pimenta dioica Seed Extracts

Abstract

Silver Nanoparticles were prepared biogenetically using aqueous extract of seeds of Pimenta dioica. Different volumes of extract were added to prepare formulations named AgNP-1, AgNP-2 and AgNP-3. The naoparticles are confirmed to observe the color changes can observed at 420nm in UV Visible spectrophotometer. The so-formed silver nanoparticles have been evaluated for in-vitro antimicrobial activity against S. aureus, P. vulgaris, B. subtilis. The produced silver nanoparticles were found to be effective against all three bacteria. Significant activity was shown with the formulation AgNP-3 and AgNP-2 compared to standards. All the formulations exhibited good activity but comparatively higher against Bacillus and Putida. The toxicity of the silver particles against bacterial cells was hence proved. This infers that the increase in extract concentration results an increase in formation of silver nanoparticles.