Formulation and evaluation of tinospora cordifolia topical gel for antimicrobial activity

Abstract

This study investigates the formulation and evaluation of agel extract of Tinospora cordifolia, aimed at addressing diabetes mellitus and antimicrobial resistance. Tinospora cordifolia is a widely recognized medicinal plant traditionally used for its antidiabetic and antimicrobial properties. The dried powder was extracted with ethanol using a mechanical shaker for 3 hours. A topical formulation like gel containing ethanolic extract was formulated using gelling agents in different concentration ratios. These gels were evaluated for physico-chemical parameters, viscosity, spreadability, pH, and antimicrobial activity. Agar well diffusion and broth microdilution methods were employed against common pathogens, including E.coli,candidaalbicans, and staphylococcus aureus, to evaluate antimicrobial efficacy. A topical gel formulation was successfully formulated containing ethanolic content of Tinospora cordifolia. The gel showed an effect on microbial activity, particularly against E.coli. The formulated Tinospora cardifolia gel extract is promising as a dual-action topical application for treating infections and managing blood glucose levels in diabetic patients. These findings support further investigation into the clinical applications of this formulation, highlighting its potential in interrogative medicines. Additional studies are needed to elucidate the underlying mechanisms and optimize the formulation for therapeutic use.