Application of a quechers extraction method for the isolation and identification of selected pesticides by using UV- visible spectrophotometer and TLC

Abstract

Purpose: The method was developed and validated for Chlorpyrifos using UV spectroscopy with the Quechers extraction application. Method: Chlorpyrifos in the Distilled water and its absorbance were estimated using UV-visible spectrophotometry. Linearity, regression equation, accuracy, precision, standard deviation, etc., parameters were calculated and validated per ICH guidelines. chloropyrifos was determined in marketed form using these validated parameters. Results: The ? (max) of chloropyrifos in the distilled water was 289nm. With a correlation coefficient 0.999, the drug exhibits linearity within the concentration range of 1-6 ?g/ml. The method's correctness has been verified, and three distinct recovery experiment levels—50%, 100%, and 150%—were carried out. It was discovered that the recovery percentage ranged from 100.03 to 101.10%. The low values of %RSD indicated the method was precise, accurate, reproducible, and rugged. Conclusion: The above-validated method may be helpful for routine analysis of Chlorpyrifos in a pesticide.