International Journal of Experimental and Biomedical Research

Methodology, Applications and Advancements of Car-T Cell Therapy in Malignancies

2024-04-08T13:04:25+0530

Despite significant advancements in cancer therapy, traditional treatments like radiation, chemotherapy, and surgery often fall short, underscoring the need for innovative approaches like activated cell therapy. Chimeric antigen receptor T cells (CAR-T cells) represent a pioneering form of this therapy, targeting cancer with modified T lymphocytes. While CAR-T cell therapy has shown high effectiveness in hematological malignancies, its success in solid tumors has been limited due to challenges such as proliferation, stability, trafficking, and tumor environment navigation. Overcoming these hurdles is crucial for enhancing the therapy's efficacy against solid tumors. The success rate of CAR-T cell therapy can reach up to 90% in leukemia patients, illustrating its potential. However, its application is still nascent, with much to learn about its long-term effectiveness and safety. This study provides an in-depth review of CAR-T cell therapy’s accomplishments and limitations in cancer treatment and explores the potential of combining CAR-T cell therapy with other treatments.

The study of antioxidant compounds in grape seeds

2024-04-08T13:20:52+0530

This paper uses spectrometric as well as chromatographic techniques to investigate the antioxidant properties of extracts from vine seeds (Vitis vinifera L.). For the study, ten vine varieties from the years 2015, 2016, along with 2017 were chosen. Four essentially distinct spectrophotometric methods were used to measure the antioxidant activity, whereas the Folin-Ciocalteu procedures were used to determine the level of total polyphenolic compounds. The composition of fourteen antioxidants was investigated in 2015. The study's findings indicate that grape seeds have a high level of antioxidant components, with variations in content between types and years. Grape seed extracts have potential applications in nutraceuticals, health supplements, as well as food product preservation.

Formulation and evaluation of cidofovir loaded microspheres

2024-04-08T13:42:55+0530

The study aimed to produce and assess cidofovir microspheres using the emulsion solvent diffusion method. Microspheres loaded with ethyl cellulose and HPMC were prepared. FTIR analysis confirmed stability with no drug-polymer interaction, and compatibility investigations ensured formulation compatibility. SEM determined particle size, with yields ranging from 84.44% to 87.62% and drug content from 64.8% to 97.2%. Particle sizes ranged from 20 µm to 210 µm, with drug loading capacities of 55.6% to 96.8% and entrapment efficiencies of 55.5% to 89.1%. Swellability studies showed a range of 0.6 to 1.6 seconds. Formulation CM8 exhibited optimal dissolution in vitro at 62.87%. Drug dissolution data were fitted to various models, with R2 values ranging from 0.937 to 0.061. Up to 45 minutes of drug release was observed. Overall, cidofovir-loaded HPMC and ethyl cellulose microspheres showed favorable release properties under optimal conditions.